Decoding KODA production to enhance plant stress tolerance

-.Credit: Tokyo Institute of Technology

KODA, a pesticide produced in very small quantities that helps plants cope with environmental stress, has been the subject of extensive research. To enhance KODA production, researchers at Tokyo Institute of Technology have developed a new plant-based platform. Using biotechnology tools, they succeeded in expressing external genes that promote KODA production in test plant species and further improved KODA yields through incubation over time.

KODA, or 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecazienoic acid, is a pesticide belonging to the class of plant oxylipin.Oxylipin is an oxidized fatty acid, mainly derived from polyunsaturated fatty acids fatty acid Like linolenic acid, linolenic acid, rafanic acid or esters thereof. Oxylipin, including KODA, helps plants recover from stress such as physical injury and infections. Many plants naturally synthesize KODA. However, except for floating spirodela, the amount is usually small. Race Lemnapauci costata.

How can this genetic benefit of Spirodela polyrhea permeate other plants? The answer seems to be advanced biotechnology. Researchers at Tokyo Institute of Technology have developed a system to improve KODA production of plants using transgenic technology. They introduced important genes involved in enhancing KODA production in two separate plant species, Spirodela polyrhizus. Nicotiana Bensamiana, which is related to tobacco plants, and Arabidopsis thaliana.

Two key genes in Spirodela polyrhizus that improved KODA production, 9-lipoxygenase (or 9-LOX) and allenoxide synthase (or AOS), are KODA in both plants when ectopically expressed in the study species. Improved yield. However, there were interspecies variations in terms of localization of the two species of KODA. In Nicotiana benthamiana tobacco, transient expression of 9-LOX and AOS increased KODA expression in the leaves. However, in Arabidopsis, the proteins encoded by the two genes needed to be localized to the plastids, endoplasmic reticulum, or lipid droplets of the intracellular structure in order to achieve sustained KODA biosynthesis. The results of these surveys are Experimental Botany Journal..

In particular, in both test plant models, researchers were able to achieve better KODA production by incubating crude leaf extracts over time. Dr. Mie Shimojima, the corresponding author of the study, further explained their technique: “By incubating a crude extract of transgenic leaves, a specific lipid that releases α-linolenic acid, a precursor of KODA. Ensured disassembly. This has become richer in KODA over time. “

Increasing the physiological level of KODA can be a blessing for growers seeking to maintain significant yields in the face of increasing environmental variability. “Not only did we find the key to improving KODA production in plants, but we also opened the door to future research on other oxylipins in various plant species,” said Dr. Shimojima. In fact, researchers strongly believe that their system can be adapted to mass-produce a variety of oxylipins for incorporation into fertilizers and pesticides.

Further research is needed to clarify the effects of plant-produced KODA on physiology. plant.. However, a new plant-based platform for improving KODA production, including intracellular localization of key enzyme genes followed by incubation of crude products, is a welcome development for the world of plant biotechnology. It’s safe for now.

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For more information:
Yuta Ihara et al., Development of a platform for the production of oxylipin KODA in plants, Experimental Botany Journal (2021). DOI: 10.1093 / jxb / erab557

Quote: Decoding of KODA production to enhance stress tolerance of plants (February 15, 2022) from https: // Acquired on February 15, 2022.

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Decoding KODA production to enhance plant stress tolerance

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