Microgel coating helps donor cells reverse pulmonary fibrosis

Gel-coated (red) mesenchymal stromal cells (yellow) can degrade collagen (green) over a distance in the presence of tumor necrosis factor-α. Credits: Jae-Won Shin and Sing-Wan Wong / UIC

Researchers at the University of Illinois at Chicago say that treatment with microgel-coated mesenchymal cells may help relieve fibrosis and promote tissue remodeling, even after lung tissue damage. Was shown.

Pulmonary fibrosis is a chronic disease caused by environmental toxins, medications, or medical conditions such as pneumonia or rheumatoid arthritis.The feature is that it can be scratched Organization Damaged or unchecked immune responses can cause mild to severe dyspnea and oxygen deficiency. Fibrosis is now considered almost irreversible, as current medications are slightly more effective in managing symptoms and generally cause serious side effects.

Mesenchymal stromal cells (MSCs) are pluripotent and self-replicating. Stem cells, And the potential to treat conditions like fibrosis are being studied.

“Previous studies have tested the therapeutic effects of MSC, which is known to suppress inflammation and adapt to a variety of tissue environments, but the effect is so far high levels of inflammation and scar tissue still forming. It was limited to the early stages of the disease, “said Jae-Won Shin, assistant professor of pharmacology and bioengineering at the UIC School of Medicine and head of the study. “Our approach was to optimize MSC-based therapies to work after inflammation was alleviated, which is the time most people are diagnosed with fibrosis.”

As explained in a new treatise published in Natural biomedical engineering, UIC researchers have designed thin microgels that, when designed in a particular way, can increase the therapeutic potential for MSC degradation. Scar tissue Regenerate healthy tissue in a mouse model of fibrosis.

Singh and his colleagues designed a microgel that was as soft as healthy lung tissue and incorporated a small protein called tumor necrosis factor-α, also known as TNF-α, which MSC collagenase. It acts as an inflammatory signal that encourages the synthesis of. Collagenase is an enzyme that breaks down excess collagen in fibrous tissue and promotes repair of damaged tissue.

To optimize MSC with microgels, UIC researchers have designed a microfluidic device to encapsulate. Individual cells Quickly and evenly with a thin gel.

“We have downsized the individual cells that are important for the delivery of therapeutic agents to the small airways of the lungs to a small scale,” said Sing-, the lead author of the study and a UIC postdoctoral fellow in the Department of Pharmacology. Wan Wong said. Regenerative medicine.

In a model of fibrous injury, UIC researchers observed a decrease in fear indicators and an increase in health indicators. Lung tissueOnly among mice treated with MSCs coated with TNF-α integration gel via single cell encapsulation, such as normal collagen levels and architecture.

“This is one of the first scientific evidences that collagen levels can be normalized after fibrotic injury, and that the cellular environment as well as the cellular environment can be normalized. cell “Our results suggest a viable approach for predictively programming cell function to achieve the desired therapeutic outcome,” says Shin.

Studies suggest that targeting mechanically sensitive proteins may treat pulmonary fibrosis

For more information:
Sing Wan Wong et al, Inhibition of abnormal tissue remodeling by mesenchymal stromal cells monolayer-coated with softgels, which presents defined chemical-mechanical clues, Natural biomedical engineering (2021). DOI: 10.1038 / s41551-021-00740-x

Quote: Microgel coating boosts donor cells to reverse pulmonary fibrosis (June 8, 2021) Obtained from June 8, 2021

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Microgel coating helps donor cells reverse pulmonary fibrosis

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