Researchers get more efficient red bioluminescence than those on the market

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Developed in collaboration with Japanese researchers, it produces brighter, longer-lasting far-red light. This innovation can be used to image cells and tissues for diagnostics and biomedical research. credit: International Journal of Molecular Science

Researchers at the Federal University of San Carlos (UFSCar) in the state of Sao Paulo, Brazil, have developed a new far-red luciferin-luciferase system that is more efficient than the ones on the market. Articles on this subject International Journal of Molecular Science..

This study focuses on the theme project “Arthropod Bioluminescence: Biodiversity, Biochemical Origins in the Brazilian Biome, Structural / Functional Evolution of Luciferases, Molecular Differentiation of Lanterns, Biotechnology, Environment, Education” Supported by the Sao Paulo Research Foundation-FAPESP through “Applications”. The main researcher is Vadim Viviani, a biochemist and professor at UFS Car.

“We have acquired a new luciferin-luciferase system that produces far-red light at a wavelength of 650 nanometers and emits the brightest bioluminescence ever reported in this part of the spectrum. It is a very promising result for bioluminescence imaging of biological and pathological processes, “said Bibiani.

Luciferase is an enzyme that catalyzes the oxidation of luciferin, a compound found in some animals, algae, and fungi. Oxidation is the cause of the phenomenon of bioluminescence, which consists of the emission of light with wavelengths in the blue to red range.

The firefly luciferin-luciferase system is widely used for creating images of cell cultures and living animal models. For example, it helps doctors monitor metastases and see how the tumor responds to treatment. It is also used to track the virus infection process and the impact of candidate drugs on viruses such as the new coronavirus.

“Hemoglobin, myoglobin, and melanin absorb very little long-wavelength light, so red bioluminescence is preferred when imaging biological or pathological processes in mammalian tissues. Detection is above all. Optimal in the far-red and near-infrared bands, but there is no bioluminescent system light that naturally emits far-red. “

“Several transgenic forms of luciferase and synthetic analogs of natural luciferin are commercially produced. Combined to produce light with wavelengths as long as 700 nanometers, produced by these man-made systems. The light produced is generally much weaker and shorter-lived than light from the natural bioluminescent system. “

Viviani and co-workers used genetic engineering to modify luciferase from the railroad worm Phrixothrix hirtus, the only luciferase that naturally emits red light, cloned by Viviani 20 years ago. This was combined with a luciferin analog synthesized by a colleague at the University of Electro-Communications in Tokyo, Japan. The result is a much more efficient far-red luciferin-luciferase system.

“Our best combination is much redder at 650 nanometers, three times brighter than natural luciferin and luciferase, and about 1,000 times brighter than the same luciferase using a commercially available analog,” Viviani said. I will.

“In addition to long wavelengths and intense brightness, our combination has better thermal stability and cell membrane permeability. Above all, it produces and decays more persistent continuous bioluminescence. It takes at least an hour to significantly facilitate real-time imaging of biological and pathological processes. ”

Researchers show how railroad worms produce red light

For more information:
Vadim R. Viviani et al, a combination of engineering railroad worm luciferase and a very bright far-red bioluminescent luminescence based on a 6′-amino analog for bioimaging purposes, International Journal of Molecular Science (2020). DOI: 10.3390 / ijms22010303

Quote: Researchers are commercially available products obtained from on March 30, 2021 (March 30, 2021). ) Get more efficient red bioluminescence.

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Researchers get more efficient red bioluminescence than those on the market

Source link Researchers get more efficient red bioluminescence than those on the market

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